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Volume 3 No. 4, October 2008 |
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A Study of the Inhibitory Role of Ghrelin Infusion on the Pancreatic Exocrine Secretion in Male Albino Rats Romysa A. El-Sherbeny & Ghada M. Abou Fard
Department of Physiology, Faculty of Medicine, Tanta University,
Egypt
Aim: The present work was done to investigate the inhibitory role of
ghrelin infusion on the pancreatic volume and protein secretion in
anaesthetized male albino rats. Materials & Methods: The rats were
divided into 4 equal groups: First group was the control group.
Second group: Pancreatic secretion was stimulated by infusion of
wheat germ lectin, which is known to be a stimulus of
cholicystokinin hormone release, preceded by ghrelin infusion and
continued for one hour and after acute subdiaphragmatic vagotomy.
Third group: was infused by 2-D-glucose, which acts as central vagal
stimulant, preceded by ghrelin infusion and continued for one hour
Fourth group: was infused by bethanechol, which is a cholinergic
receptor agonist, preceded by ghrelin infusion and continued for one
hour. Then the pancreatic secretion was collected after
anaesthetizing male albino rat, through a cannula inserted in the
common bile duct. Results: Significant increase of the pancreatic
protein content by wheat germ lectin infusion, through stimulation
of cholicystokinin which stimulated pancreatic secretion, Ghrelin
infusion caused significant inhibition of protein secretion and this
inhibition was continued after acute subdiaphragmatic vagotomy,
Ghrelin also caused significant inhibition of the pancreatic protein
secretion, which caused by central stimulation of vagus nerve by
2-D-glucose infusion. Ghrelin showed significant inhibition of the
pancreatic protein secretion which caused by bethanechol, that
acting as a muscarinic receptor agonist. Conclusion: Ghrelin is a
potent inhibitor of pancreatic exocrine protein secretion and the
mechanism of its action may be directed at the level of the intra
pancreatic neurotransmission.
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