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Volume 3 No. 4, October 2008 |
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Immunohistochemical Study of the Prevalence of EBV- Antigen in
Nasopharyngeal Carcinoma Departments of Pathology, Faculty of Medicine, Tanta University, Egypt
Tanta Med. Sc. J 2008; 3(4):126-136
Background/Aim: A broad range of neoplasms can arise in the
nasopharynx derived from epithelial, lymphoid, mesechymal,
neurogenic, and rarely from embryonic remnant. These tumours have a
long latent period with few primary symptoms which often lead to
delay in there diagnosis. This retrospective study was designed for
highlighting the challenges involved with the recognition of the
nature of nasopharyngeal carcinoma, their classification, and
correlation of the available clinical data with histopathological
and immunohistochemical diagnosis and the prevalence of EBV
infection among the studied cases of NPC. Patients & Methods: The
present work was carried out on 23 cases referred as biopsy from
nasopharyngeal tumour to Pathology Department, Faculty of Medicine
Tanta university, Tanta Cancer center, the private laboratory during
the last five years started from January 2002 and ended on December
2006 .Tissue specimens were all obtained by bunch and excision
biopsy. Available clinical data were tabulated and analysed. All
cases were subjected to routine histopathological examination and
immunohistochemical study for EBV-LMP1. Results: In the present
study UNPC was more common (69.6 %) than keratinizing NPC (30.4 %).
The former occurred in younger patient (9/16), meanwhile the latter
occurred in older individuals (5/7). NPC was common in male (60.9 %)
and bimodal distributions of cases were detected. The diagnosis of
UNPC in most cases could be established through the presence of
atypical epithelial cells and the lack of cytological atypia among
reactive lymphocytes, as well as by immunohistochemical examination
for EBV-LMP1. In the present study IHC was employed on paraffin
embedded specimens of all the studied cases of NPC using of EBV-LMP1
reactivity. Accordingly, it was found that positive strong
cytoplasmic and /or membranous immunorectivity for EBV-LMP1 was
evident in (69.6%) of the total cases of NPC, while (23.4 %) were
negative. Positive cases included (57.2%) of keratinizing NPC, and
(75%) of UNPC, meanwhile negative immunostaining for EBV-LMP1 was
seen in the remaining (42.8%) of keratinizing NPC and the remaining
(25%) of UNPC. Conclusion: The overall association of EBV-LMP1
immunoreactivity with NPC was found to be statistically significant
with higher percentage of positive association among UNPC cases.
Correlation of EBV-LMP1 immunoreactivity with age and gender among
cases of NPC was found to be statistically insignificant.  |
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