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Lactic dehydrogenase
and Catalase Enzymes Activities in Bovine Fascioliasis
Ghada Fouad El-Baradey(1) & Soad S. Abd El-all El-Gaby(2)
Department of Anesthesiology &ICU, Faculty of Medicine, Tanta
University(1) & Department of Anesthesiology &ICU, Faculty of
medicine for girls AL-Azhar University(2), Egypt.
Tanta Med. Sc. J 2009; 4(3):70-82
ICID: 907312
Article type: Original articleIC™ Value: 4.54
| Abstract provided by Publisher |
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Back ground / Aim: Lactic dehydrogenase (LDH) and catalase (CAT)
are group of oxidoreductases enzymes catalyzing biological
oxidation/reduction reactions. They are widely used in clinical
diagnosis and other analytical applications. Fasciolosis is an
important helminth disease affected human and animal. The present
work was designed to investigate of the effect of fasciolasis &
fasciolicides drugs: rafoxinide and triclabendazole in hepatic LDH
and CAT. Materials & Methods: Kinetic studies of the purified LDH
and CAT were determined at various concentrations of substrate in
the absence and presence of the fasciolicides drugs rafoxinide and
triclabendazole. Results: Lactate dehydrogenase (LDH) from
Fasciola-infested cows and buffalos showed a significant increase in
its activity compared to control. Both drugs have inhibitory action
on the enzyme from the control and Fasciola - infested cows.
Rafoxinide showed the pattern of the competitive type inhibition,
whereas triclabendazole showed that of the mixed-type one. On the
other hand, catalase from Fasciola-infested cows and buffalos
displayed a non-significant change in its activity compared to
control. Conclusion: LDH may be used as a biomarker for fasciolosis
and its treatment.
ICID 907312
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