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The Role of Nitric
Oxide in Ischemia-Reperfusion Injury of the Pancreas in Adult Albino
Rat: Histological, Immuno-Histochemical and Morphometric Study
Manal I. El-Bermawy
Department of Anatomy - Faculty of Medicine - Tanta University,
Egypt
Tanta Med. Sc. J 2008; 3(3):48-65
Article type: Original article
Background/Aim: Ischemic–reperfusion injury, thus
graft degeneration remains a major problem as post-operative
management of organ transplantation. Also, ischemic injury to
pancreas is known to occur in various clinical setting as, cardiac
surgery, hemorrhagic shock and organs transplantation. This study
was carried out to clarify the effect of nitric oxide (NO) drug
(sodium nitroprusside) on the ischemic–reperfusion injury of the
pancreas in adult albino rat, using histological,
immuno-histochemical and morphometric study. Material & Methods: In
this study, forty adult albino rats (regardless sex) were used. The
animals were divided into two groups, a control and experimental
groups. The control group was 15 rats and the experimental group was
25 rats. The latter group was exposed to pancreatic ischemia for an
hour, after that subdivided into 5 subgroups, A, B ,C, D, E (5 rats
each). In the first three subgroups, rats were allowed to pancreatic
tissue reperfusion for 0, 24, and 48 hours respectively. While, in
the last two subgroups rats were allowed to pancreatic tissue
reperfusion for 24, 48 hours respectively associated with
intra-peritoneal injection of sodium nitroprusside 0.3mg / kg /dose
in 3 doses at 1,2,3 hours of reperfusion time. Specimens of pancreas
of all groups were taken and prepared for histological,
immuno-histochemical and morphometric study. Results: The
experimental group: Subgroup (A); revealed no apparent histological
or immuno-histochemical changes in pancreatic tissue. Subgroup (B)
and subgroup (C); after 24 &48 h. reperfusion, signs of pancreatic
tissue degeneration were obvious with marked reduction in
functioning ß-cell until it became completely negative to insulin
hormone reaction after 48h. reperfusion. Subgroup (D) and subgroup
(E); showed marked regression in all signs of pancreatic tissue
degeneration with marked increased positive immuno- histochemical
reaction to insulin hormone in beta cells. Morphometric results:
detected statistical significant changes in the average surface area
of islets of Langerhans between the experimental different
subgroups. Conclusion: This experimental study tried to demonstrate
that NO had a protective role in ischemic-reperfusion injury of the
pancreas. Therefore, administration of NO should be recommended for
clinical cases in organs transplantation with good observation of
the blood pressure.
ICID 871173
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