Volume 3 No. 3, July 2008

Lipid Profile and Paraoxonase Enzyme Activity in Obese Saudi Population
Zainy  Banjr

Department of Clinical Biochemistry, Faculty of Medicine, King Abdul-Aziz University, Jeddah, Kingdom of Saudi Arabia
Tanta Med. Sc. J 2008; 3(3):109-116
Article type: Original article

Aim: It is an investigation of the distribution PON-1 192 polymorphisms and activity and lipid profiles in 71 obese Saudi patients and 69 healthy controls. Patients & Methods: Polymerase chain reaction–based restriction fragment was used to determine PON-1 192 gene polymorphism. PON activity was measured by spectrophotometric assay. Serum lipid was measured enzymetically. Results: Fasting blood sugar was normal. Total cholesterol, triglycerides, LDL serum level were significantly higher in patient than control meanwhile HDL and APOA-1 were significantly low in patients. No significant deference was found in distribution of PON-1 192 polymorphism (QQ, RR and QR) between patients and control subjects. PON-1 192 enzyme activity was significant lower in diabetics than in control subjects. PON-1 192 RR homozygote had significantly low PON-1 192 activity than QQ and QR genotypes among the NIDDM populations (p<0.0001). Conclusion: our results suggest that the paraoxonase activities are affected by PON1 genetic variability in obese patients and controls.

ICID 874412