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Immunohistochemical Nuclear Staining For P53, PCNA, and Ki-67 in
Different Histologic Varients of Basal Cell Carcinoma
Iman El Maadawy, Abeer Hodeib, Afaf El Shafaey*, Ameena Abd Alrahman
Dermatology &
Venereology and Pathology Departments*, Faculty of Medicine, Tanta
University, Egypt.
Tanta Med. Sc. J 2007; 2(3):129-146
Article type: Original article
Background/Aim: Structural alternation of P53
gene is a common genetic change associated with basal cell carcinoma
(BCC). Proliferation antigens are expressed in the nuclei of cell
during specific stages of the cell cycle including proliferating
cell nuclear antigen (PCNA) and Ki-67. Studies have suggested that
PCNA and Ki-67 are useful diagnostic tools to differentiate benign
from malignant neoplasm and useful prognostic markers in malignant
neoplasms. The aim of this work is to detect the expression of P53,
PCNA, and Ki-67 in different histologic variants of BCCs, to study
the probability of using such markers as diagnostic and prognostic
tools. Patients & Methods: Thirty patients with different histologic
variants of BCCs were diagnosed histopathologically. Seven skin
biopsies were taken as controls. Immnunohistochemical staining for
P53, PCNA, and Ki-67 detection. Results: There is variable degree of
positivity of P53, PCNA, and Ki-67 with different histologic
variants of BCCs. There is high significant relation between
percentage of their positive cells and both aggressiveness and
recurrence of BCCs. There is positive relation between P53 and PCNA
expression. PCNA expression is greater than Ki-67 in all studied
variants except for the superficial variants, which were negative
for P53 expression. Conclusion: Both the tumor suppressor gene P53
and the proliferation markers; PCNA and Ki-67 are expressed in BCCs.
They are reliable prognostic markers for aggressive behavior of BCCs.
They are valuable tool in prediction of possible recurrence. Their
staining appeared to be superior to traditional histologic features
in predicting clinical recurrence in primary BCC's and further
prospective studies in a larger patient group are warranted. |